Phase I
Immunization |
- MonoBoost™ protocol or conventional protocol (4months longer)
- ImmunoPlus Technology
- DNA Immunization
- Animals: BALB/c mice, C57BL6 mice, or rats
- Number of animals immunized
|
- Test bleed report
(optional: total serum protein delivery)
|
- Modify immunization strategy
- Proceed with selected animals for fusion
|
8-10 |
Phase II
Cell Fusion and Screening |
- Indirect ELISA screening and WB validation
- Capture ELISA
- Competitive ELISA
- FACS screening
- Counter screening
- Additional screening options available
|
- Parental supernatant report or 2 mL supernatants
|
- Alter screening methodology
- Select ~5-10 clones which proceed to subcloning
|
5-7 |
Phase III
Subcloning, Screening, and Expansion |
- Subclone Screening
- Cryopreservation
- Cell banking
- Roller Bottle Production
- Antibody Purification
|
- 2 vials of up to 10 hybridoma cell lines
- 5 mL sub-clone supernatant
- Purified Antibodies
|
- FREE cell banking at GenScript for 6 months
- Scale-up production
- Continue with other services: antibody screening, rAb production, etc.
|
4-7 |
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