서비스 > Protein Expression & Immunological

Recombinant mammalian protein expression

- Recombinant protein의 post-translational modification 문제를 해결한 mammalian expression platform
- Therapeutic protein의 functional test & characterization
- HEK293 cell-base
(기본제공)
- Small scale expression pilot test
- Large scale (기본 1 Liter) protein expression & purification
- Scale-up to reach desired protein amount (기본 100~200μg)
- Affinity purification
- Purity analysis and quantification
- Tag removal as requested

(Option)
- Codon optimization and gene synthesis
- Sub-cloning of expression vector
- DNA preparation and Q.C.

(Option)Gene synthesis

- Codon optimization

codon
Step 1
(Option)Expression vector sub-cloning

- Vector design/cloning

expression
Step 2
Small scale test

-Transient transfection
- Small scale test

smallscale
Step 3
Large scale

- Transient transfection
- Large scale culture

Transduction
Step 4
Purification

- Affinity column
- SDS-PAGE purity analysis

Mitochondria
Step 5